C11orf71

Gene

C11orf71 gene, also known as URLC7(up- regulated in lung cancer 7), is a protein coding gene found in Homo sapiens found on the negative strand. The gene consists of 1980 base pairs and one exon and encodes for the mRNA transcript that translates the protein for isoform 1.

Transcript

C11orf71 has over 1.6 times the average expression of a standard gene and it is functionally important in the brain, testis, liver and heart and less prominent but still present in other tissues. This expression is conserved over some of its orthologs, specifically olive baboons and old world monkeys. The 3 prime UTR is much longer than the 5 prime UTR and contains three polyadenylation sites.

Protein

Human C11orf71 encodes isoform 1 , consisting of 123 amino acids. The molecular weight of Human C11orf71 protein is 13 kilodaltons and the predicted isoelectric point is 12. The average human proteome isoelectric point is 6.71 so an isoelectric point of 12, which was found in the C11orf71 protein, is significantly higher and more basic. C11orf71 was rich in Serine and Arginine and poor in lysine, threonine, cysteine, and tryptophan.

Figure4: I Tasser Ribbon coloration in uniform. Both contain 11 highlighted portions representing most conserved amino acids.  

Protein Structure

Human C11orf71 is predicted to have two helices located throughout the protein.

Protein Localization

Human C11orf71 is predicted to be localized within the nucleoplasm and nuclear body of the cell.

Protein - Protein Interaction

Protein TPP2, GRPEC58, DKK1, and prfB all are found to have physical association with C11orf71 which lead to a confidence level above 0.35. MNAT1,USP34,NACC2, although associated with the protein, are speculated to not have physical association. All proteins were located within the cytoplasm and nucleus where C11orf71 is found.

Evolution of C11orf71

No paralogs were found. Human gene C11orf71 was first found 24 million years ago in primate and then was not found again until 87 million years ago in rodents. Between 94-100 million years ago, the gene was found in many other placental mammals such as carnivores and cetacea.

Orthologs:

genus species	common name	taxon	date of div MYA	accession #	seq length AA	seq ID %	seq sim %	CorSeqDiv%	CorSeqDiv%
Homo sapiens	Humans	Primates	0	NP_001258491.1	123	100	100	0	0
Macaca multatta	Indochinese rhesus macaque	Primates	28.2	XP_014971244.1	127	88	91	12.78333715	12.8
Oryctolagus caniculus	European rabbit	Lagomorpha	87	XP_002708469.1	128	66	75	41.5515444	41.6
Ochontona curzoniae	Plateau pika	Lagomorpha	87	XP_040833205.1	104	52	61	65.39264674	65.4
Castor canadensis	North American beaver	Rodentia	87	XP_020036644.2	115	72	78	32.8504067	32.9
Marmota flaviventris	Yellow-bellied marmot	Rodentia	87	XP_027786394.2	119	60	67	51.08256238	51.1
Suncus etruscus	Etruscan shrew	Eulipotyphla	94	XP_049634650.1	110	43	58	84.39700703	84.4
Galemys pyrenaicus	Pyrenean desman	Eulipotyphla	94	KAG8524576.1	131	62	70	47.80358009	47.8
Artibeus jamaicensis	Jamaican fruit-eating bats	Chiroptera	94	XP_053526410.1	115	53	62	63.48782724	63.5
Myotis myotis	Bat	Chiroptera	94	XP_036179868.1	119	55	63	59.78370008	59.8
Orcinus orca	Orca	Cetacea	94	XP_049569180.1	131	65	71	43.07829161	43.1
Delphinus delphis	Short beaked doplphin	Cetacea	94	XP_059873910.1	131	67	72	40.04775666	40.1
Balaenoptera musculus	Blue whale	Cetacea	94	XP_036717480.1	131	64	71	44.62871026	44.6
Kogia breviceps	Sperm whale	Cetacea	94	XP_058926189.1	131	66	72	41.5515444	41.6
Phoca vitulina	Harbor seal	Carnivora	94	XP_032283947.1	131	70	78	35.66749439	35.7
Moschus berezovskii	Dwarf musk deer	Artiodactyla	94	XP_055279029.1	132	68	76	38.56624808	38.6
Equus asinus	African wild ass	Perissodactyla	94	XP_044608542.1	131	72	77	32.8504067	32.9
Mustela erminea	Stoat	Carnivora	94	XP_032213699.1	131	69	77	37.10636814	37.1
Orycteropus afer afer	Aardvark	Tubulidentata	99	XP_007934792.1	131	63	70	46.20354596	46.2
Chrysochloris asiatica	Cape golden mole	Afrosoricida	99	XP_006834040.1	131	68	74	38.56624808	38.6

Post translational modifications

Predicted protein graphic of human C11orf71

C11orf71 contains 5 speculated phosphorylation sites that are conserved amongst orthologs. Phosphorylation sites were found at amino acids 9, 65, 90 ,92 , and 122.

C11orf71 contains one predicted O - (alpha) - GIcNAc glycosylation site at amino acid 74 which is a serine.

Conceptual translation of human C11orf71

See Conceptual translation for all predicted modifications and known sites.

Conditions for expression

Conditions:

House dust mite extract effect on a bronchial epithelial cell line.

H292 cells, also known as a human lung carcinoma cell line, were soaked in house dust mite and then microarrayed in order to identify gene expression. It was concluded that epithelial cells may produce genes that can respond to the environment.  When house dust mite extract was added, C11orf71 was down-regulated by 25 percent.

Fetal and adult reticulocytes

RNA from blood reticulocytes was used in order to understand and identify what genes are transcribed of erythrocytes maturing. When adults contain mature red blood cells, the presence of the gene C11orf71 on average decreases between 30-40 percent.

Tumorigenic breast cancer cell

Gene expression was analyzed in normal, non tumorigenic and tumorigenic cancer cells. Breast cancer has high expression of CD44 and low expression of CD24. Research then compared the genes that were expressed between the two. C11orf71 was on average under expressed by 25 percent.

References

This article "C11orf71" is from Wikipedia. The list of its authors can be seen in its historical and/or the page Edithistory:C11orf71. Articles copied from Draft Namespace on Wikipedia could be seen on the Draft Namespace of Wikipedia and not main one.

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