Gas Chromatography-UV Spectrometry

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Example of a GC-UV instrument

Gas Chromatography-UV Spectrometry (GC-UV) is a full scan analytical method that combines gas chromatography with UV Spectrometry. It is a combination of techniques, called hyphenated techniques. Chromatography is an analytic chemistry technique widely used in laboratories as a scientifically proven method for gaining chemical information. GC-UV is in the same category as gas chromatography–mass spectrometry (GC-MS) and GC-FTIR.

History

The GC-UV method is based on the experiences in analytical chemistry. In the 1960s, the research of the Lagesson couple, Ludmila Lagesson-Andrasko and Verner Lagesson, discovered and tested the method in theoretical studies.

Difference between GC-MS and GC-UV

Mass spectroscopy is being used worldwide as the golden standard method. The disadvantages of the MS-method is that it has the ability to disassemble the gas molecules. The GC-UV method is non-destructive and is not being affected by temperature, pressure or elution because of the spectral absorption of light that determines the substances. The GC-UV-method is based on the additional use of UV-light absorption. Together with UV-light a chromatograph can see down into a low wavelength of the spectra. Due to this advantage of UV light absorption, the analysis of substances can be conducted with high sensitivity. No spectral shifts, like with liquid chromatography, are present and therefore no cut off wavelength are present due to any liquid solvent. By conducting chemical analysis, the addition of UV-light absorption gives the reader more qualitative information about the substance, non-destructive.

A GC-UV Chromatograph can be made small and lightweighted and therefore is useful in numerous application areas. The principle of spectrophotometry, called Lambert Beers Law, is the most reliable method. GC-UV analysis can be used to give quantitative information based on the light absorbing power of the compound. The method also gives qualitative information of isomers, compounds and chemical identification of several compounds.

When using GC-UV analysis the unknown substance can be identified both qualitatively and quantitatively. A GC-UV instrumentation is relatively light, mobile and compact making it easier to have GC-UV analysis outside laboratories. The GC-UV method can detect, identify and classify unknown substances eluting from a gas chromatograph and can be monitored. Selective analysis can be conducted. Inorganic as well as organic compounds can be identified.

Applications

• Breath gas analysis
• Alternative medicine
• Pharma
• Forensics
• Environmental science
• Agrochemical
• Flavors & fragrances
• Food & beverages
• Speciality chemicals
• Petrochemical
• Fuels refining
• Containers
• Explosives
• Narcotics
• Spaceflight instrumentation

See also

• Gas chromatography–vacuum ultraviolet spectroscopy
• Capillary electrophoresis–mass spectrometry
• Liquid chromatography–mass spectrometry
• Ion-mobility spectrometry–mass spectrometry
• Prolate trochoidal mass spectrometer
• Pyrolysis–gas chromatography–mass spectrometry

References

• WORKMANJR, J (2001), "Practical Guide for Evaluating Ultraviolet Spectra", The Handbook of Organic Compounds, Elsevier, 1, pp. 65–67, doi:10.1016/b978-012763560-6/50011-8, ISBN 978-0-12-763560-6, retrieved 2021-04-04
• Lagesson-Andrasko, Ludmila; Lagesson, Verner; Andrasko, Jan (March 1998). "The Use of Gas-Phase UV Spectra in the 168−330-nm Wavelength Region for Analytical Purposes. 1. Qualitative Measurements". Analytical Chemistry. 70 (5): 819–826. doi:10.1021/ac971009v. ISSN 0003-2700. PMID 21644613.
• Leanderson, Per; Lagesson, Verner; Tagesson, Christer (September 1997). "Demonstration of Nitric Oxide on Asbestos and Silicon Carbide Fibers with a New Ultraviolet Spectrophotometric Assay". Environmental Health Perspectives. 105: 1037–1040. doi:10.2307/3433505. ISSN 0091-6765. JSTOR 3433505. PMC 1470179. PMID 9400696.
• Jones, A.W.; Lagesson, V.; Tagesson, C. (October 1995). "Determination of isoprene in human breath by thermal desorption gas chromatography with ultraviolet detection". Journal of Chromatography B: Biomedical Sciences and Applications. 672 (1): 1–6. doi:10.1016/0378-4347(95)00207-y. ISSN 0378-4347. PMID 8590920.
• "Masthead". Biomedical Chromatography. 9 (2): fmi. March 1995. doi:10.1002/bmc.1130090201. ISSN 0269-3879.
• Hatzinikolaou, Dimitris G.; Lagesson, Verner; Stavridou, Anastasia J.; Pouli, Aristea E.; Lagesson-Andrasko, Ludmila; Stavrides, John C. (July 2006). "Analysis of the Gas Phase of Cigarette Smoke by Gas Chromatography Coupled with UV-Diode Array Detection". Analytical Chemistry. 78 (13): 4509–4516. doi:10.1021/ac052004y. ISSN 0003-2700. PMID 16808460.
• Nelson, Nina; Lagesson, Verner; Nosratabadi, Ali Reza; Ludvigsson, Johnny; Tagesson, Christer (September 1998). "Exhaled Isoprene and Acetone in Newborn Infants and in Children with Diabetes Mellitus". Pediatric Research. 44 (3): 363–367. doi:10.1203/00006450-199809000-00016. ISSN 0031-3998. PMID 9727714.
• Nilsson, Anders; Lagesson, Verner; Bornehag, Carl-Gustaf; Sundell, Jan; Tagesson, Christer (October 2005). "Quantitative determination of volatile organic compounds in indoor dust using gas chromatography-UV spectrometry". Environment International. 31 (8): 1141–1148. doi:10.1016/j.envint.2005.04.003. ISSN 0160-4120. PMID 15936080.
• Friberg, Jonas (February 2014). "Volatile organic compounds analyzed by gas chromatography-deep ultraviolet spectroscopy". Acupuncture and Related Therapies. 2 (1): 25–28. doi:10.1016/j.arthe.2013.12.001. ISSN 2211-7660.
• Singh, Parmjit; Namarach, Kutana; Rashed Salem, Wedad; Shahzadi, Khansa (2018-08-09). "The current and potential future uses of plateletrich plasma in orthodontics". Annals of Dentistry and Oral Health. 1 (1). doi:10.33582/2639-9210/1004. ISSN 2639-9210.
• Snow, Nicholas H. (2006), "Chapter 14 Gas chromatography", Modern Instrumental Analysis, Comprehensive Analytical Chemistry, Elsevier, 47, pp. 443–483, doi:10.1016/s0166-526x(06)47014-8, ISBN 978-0-444-52259-7, retrieved 2021-04-04
• Andrasko, Jan; Lagesson-Andrasko, Ludmila; Dahlén, Johan; Jonsson, Bengt-Harald (July 2017). "Analysis of Explosives by GC-UV". Journal of Forensic Sciences. 62 (4): 1022–1027. doi:10.1111/1556-4029.13364. PMID 28070907.
• Khan, Sulaiman; Newport, David; Le Calvé, Stéphane (2019-11-28). "Gas Detection Using Portable Deep-UV Absorption Spectrophotometry: A Review". Sensors. 19 (23): 5210. Bibcode:2019Senso..19.5210K. doi:10.3390/s19235210. ISSN 1424-8220. PMC 6929016 . PMID 31795069.
• Lagesson, H. V.; Nilsson, A.; Tagesson, C. (November 2000). "Qualitative determination of compounds adsorbed on indoor dust particles using GC-UV and GC-MS after thermal desorption". Chromatographia. 52 (9–10): 621–630. doi:10.1007/bf02789762. ISSN 0009-5893.

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