Ke Zhang Reid
Ke Zhang Reid is a biochemist whose main research focuses on the mechanism of epigenetic regulation. She works in the Wake Forest University biology department.
Education
Zhang was born in China, where she finished her undergraduate degree (1993-1997) in Wuhan University and her master’s program (1997-1999) in Peking Union Medical College, which is a Chinese Ministry of Education Double First Class University Plan university.[1]. She then moved to the US and attended the University of Texas for her doctoral degree (1999-2005), where she focused on how the Set1 methyltransferase opposes Ipl1 aurora kinase functions in chromosome segregation[2].
Career
Zhang worked in the National Cancer Institution and National Institutes of Health as a postdoctoral fellow for six years (2006-2012). After this, she joined the Wake Forest University biology department as an associate professor and a research mentor.
Past Research
In her doctoral research, she concluded that the balance in activities is modulated by the Set1 methyltransferase. The deletion of SET1 suppresses chromosome loss in ipl1-2 cells. She also found that Set1 has important, unexpected functions in mitosis.
During her postdoctoral fellowship, she published six journals focusing on retrotransposon Tf1 being targeted to Pol II promoters by transcription activators[3], the role of the Clr4 methyltransferase complex in nucleation[4], cell cycle control of centromeric repeat transcription and heterochromatin assembly[5], conservation and rewriting of functional modules revealed by an epistasis map in fission yeast[6], histone H2A.Z cooperation with RNAi and heterochromatin factors to suppress antisense RNAs[7], and RNAi-independent nucleation of heterochromatin revealed by defects in RNA quality control factors[8].
After she joined Wake Forest University, she published six journals focusing on RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation[9], a novel epigenetic silencing pathway involving the highly conserved 5’-3’ exoribonuclease Dhp1/Rat1/Xrn2 in Schizosaccharomyces pombe[10], the fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis[11], loss of elongation-like factor 1 spontaneously induces diverse, RNase H-Related suppressor mutations in Schizosaccharomyces pombe[12], a deep-sequencing-assisted, spontaneous suppressor screen in the fission yeast Schizosaccharomyces pombe[13], the Catalytic-Dependent and independent roles of Lsd1 and Lsd2 lysine demethylases in heterochromatin formation in Schizosaccharomyces pombe[14].
Current research
Epigenetic mechanisms that modify DNA or chromatin play fundamental roles in the control of gene expression. The Zhang-Reid lab uses genetics, cell biology, and genomic strategies to study the epigenetic mechanisms that regulate eukaryotic gene expression and genome integrity, using fission yeast as a model organism. The three main areas of research in the Zhang-Reid laboratory investigate epigenetic mechanisms in control of gene expression, meiotic gene silencing during mitosis and regulated expression during meiosis, and RNA-mediated genome instability.
Zhang focuses on the mechanisms of epigenomic regulation. One of the regulations involves how ncRNAs are produced by RNAPII and how the dysregulation of ncRNA can cause cancer. Another focus of her research is trying to figure out how lysine methylation and deacetylation of histones and non-histone substrates govern cellular functions. In one of her recent studies, her team found that two of the lysine-specific-demethylases, Lsd1 and Lsd2, repress heterochromatic transcripts both depend on and independent of their catalytic activities. Lsd1 and Lsd2 can also regulate one another and cooperate with other histone modifiers for gene silencing[15]
References
- ↑ "Peking Union Medical College". January 23, 2022 – via Wikipedia.
- ↑ Zhang, Ke; Lin, Wenchu; Latham, John A.; Riefler, Gary M.; Schumacher, Jill M.; Chan, Clarence; Tatchell, Kelly; Hawke, David H.; Kobayashi, Ryuji; Dent, Sharon Y. R. (September 9, 2005). "The Set1 methyltransferase opposes Ipl1 aurora kinase functions in chromosome segregation". Cell. 122 (5): 723–734. doi:10.1016/j.cell.2005.06.021. PMC 1794220. PMID 16143104.
- ↑ Ye, Leem; Tl, Ripmaster; Fd, Kelly; H, Ebina; Me, Heincelman; K, Zhang; Si, Grewal; Cs, Hoffman; Hl, Levin (April 11, 2008). "Retrotransposon Tf1 is targeted to Pol II promoters by transcription activators". Molecular Cell. 30 (1): 98–107. doi:10.1016/j.molcel.2008.02.016. PMC 2423209. PMID 18406330 – via pubmed.ncbi.nlm.nih.gov.
- ↑ Zhang, Ke; Mosch, Kerstin; Fischle, Wolfgang; Grewal, Shiv I. S. (April 10, 2008). "Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin". Nature Structural & Molecular Biology. 15 (4): 381–388. doi:10.1038/nsmb.1406. hdl:11858/00-001M-0000-0012-DC69-9. PMID 18345014 – via PubMed. Unknown parameter
|s2cid=ignored (help) - ↑ Chen, Ee Sin; Zhang, Ke; Nicolas, Estelle; Cam, Hugh P.; Zofall, Martin; Grewal, Shiv I. S. (February 7, 2008). "Cell cycle control of centromeric repeat transcription and heterochromatin assembly". Nature. 451 (7179): 734–737. Bibcode:2008Natur.451..734C. doi:10.1038/nature06561. PMID 18216783 – via PubMed. Unknown parameter
|s2cid=ignored (help) - ↑ Roguev, Assen; Bandyopadhyay, Sourav; Zofall, Martin; Zhang, Ke; Fischer, Tamas; Collins, Sean R.; Qu, Hongjing; Shales, Michael; Park, Han-Oh; Hayles, Jacqueline; Hoe, Kwang-Lae; Kim, Dong-Uk; Ideker, Trey; Grewal, Shiv I.; Weissman, Jonathan S.; Krogan, Nevan J. (October 17, 2008). "Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast". Science. 322 (5900): 405–410. Bibcode:2008Sci...322..405R. doi:10.1126/science.1162609. PMC 2753251. PMID 18818364.
- ↑ M, Zofall; T, Fischer; K, Zhang; M, Zhou; B, Cui; Td, Veenstra; Si, Grewal (September 17, 2009). "Histone H2A.Z cooperates with RNAi and heterochromatin factors to suppress antisense RNAs". Nature. 461 (7262): 419–422. Bibcode:2009Natur.461..419Z. doi:10.1038/nature08321. PMC 2746258. PMID 19693008 – via pubmed.ncbi.nlm.nih.gov.
- ↑ Reyes-Turcu, Francisca E.; Zhang, Ke; Zofall, Martin; Chen, Eesin; Grewal, Shiv I. S. (September 4, 2011). "Defects in RNA quality control factors reveal RNAi-independent nucleation of heterochromatin". Nature Structural & Molecular Biology. 18 (10): 1132–1138. doi:10.1038/nsmb.2122. PMC 3190054. PMID 21892171.
- ↑ M, Zofall; S, Yamanaka; Fe, Reyes-Turcu; K, Zhang; C, Rubin; Si, Grewal (January 6, 2012). "RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation". Science. 335 (6064): 96–100. Bibcode:2012Sci...335...96Z. doi:10.1126/science.1211651. PMC 6338074. PMID 22144463 – via pubmed.ncbi.nlm.nih.gov.
- ↑ Tucker, James Franklin; Ohle, Corina; Schermann, Géza; Bendrin, Katja; Zhang, Wei; Fischer, Tamás; Zhang, Ke (February 18, 2016). "A Novel Epigenetic Silencing Pathway Involving the Highly Conserved 5'-3' Exoribonuclease Dhp1/Rat1/Xrn2 in Schizosaccharomyces pombe". PLOS Genetics. 12 (2): e1005873. doi:10.1371/journal.pgen.1005873. PMC 4758730. PMID 26889830.
- ↑ Marayati, Bahjat Fadi; Hoskins, Victoria; Boger, Robert W.; Tucker, James F.; Fishman, Emily S.; Bray, Andrew S.; Zhang, Ke (September 10, 2016). "The fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis". RNA. 22 (9): 1349–1359. doi:10.1261/rna.055608.115. PMC 4986891. PMID 27365210.
- ↑ Marayati, Bahjat F.; Drayton, Alena L.; Tucker, James F.; Huckabee, Reid H.; Anderson, Alicia M.; Pease, James B.; Zeyl, Clifford W.; Zhang, Ke (August 10, 2018). "Loss of Elongation-Like Factor 1 Spontaneously Induces Diverse, RNase H-Related Suppressor Mutations in Schizosaccharomyces pombe". Genetics. 209 (4): 967–981. doi:10.1534/genetics.118.301055. PMC 6063228. PMID 29844133.
- ↑ Marayati, Bahjat F.; Pease, James B.; Zhang, Ke (March 7, 2019). "A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe". Journal of Visualized Experiments: JoVE (145). doi:10.3791/59133. PMC 7309966 Check
|pmc=value (help). PMID 30907886. - ↑ Marayati, Bahjat F.; Tucker, James F.; Cerda, David A. De La; Hou, Tien-Chi; Chen, Rong; Sugiyama, Tomoyasu; Pease, James B.; Zhang, Ke (April 13, 2020). "The Catalytic-Dependent and -Independent Roles of Lsd1 and Lsd2 Lysine Demethylases in Heterochromatin Formation in Schizosaccharomyces pombe". Cells. 9 (4): E955. doi:10.3390/cells9040955. PMC 7226997 Check
|pmc=value (help). PMID 32295063 Check|pmid=value (help). - ↑ "Ke Zhang Reid – Biology".
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