Megabarcoding
Megabarcoding is a term used to describe high-throughput specimen-based DNA barcoding, where thousands of specimens can be barcoded simultaneously for species identification and discovery [1]. This is enabled by the use of third-generation sequencing platforms including PacBio (Sequel I/II) by Pacific Biosciences and MinION, PromethION by Oxford Nanopore Technology. As compared to Sanger sequencing, megabarcoding is faster and cheaper, allowing for the large-scale generation of DNA barcodes for thousands of species [2].
Megabarcoding can help fill the dark taxa DNA barcode reference data gap for insects and accelerate species discovery[3]. This will go a long way in generating DNA reference data which is crucial for environmental DNA-based biodiversity monitoring.
References
- ↑ Chua, Physilia Y. S.; Bourlat, Sarah J.; Ferguson, Cameron; Korlevic, Petra; Zhao, Leia; Ekrem, Torbjørn; Meier, Rudolf; Lawniczak, Mara K. N. (10 March 2023). "Future of DNA-based insect monitoring". Trends in Genetics. doi:10.1016/j.tig.2023.02.012. PMID 36907721 Check
|pmid=value (help). Unknown parameter|s2cid=ignored (help) - ↑ Hebert, Paul D. N.; Braukmann, Thomas W. A.; Prosser, Sean W. J.; Ratnasingham, Sujeevan; deWaard, Jeremy R.; Ivanova, Natalia V.; Janzen, Daniel H.; Hallwachs, Winnie; Naik, Suresh; Sones, Jayme E.; Zakharov, Evgeny V. (27 March 2018). "A Sequel to Sanger: amplicon sequencing that scales". BMC Genomics. 19 (1): 219. doi:10.1186/s12864-018-4611-3. PMC 5870082. PMID 29580219.
- ↑ Fernandez-Triana, Jose L. (25 February 2022). "Turbo taxonomy approaches: lessons from the past and recommendations for the future based on the experience with Braconidae (Hymenoptera) parasitoid wasps". ZooKeys (1087): 199–220. doi:10.3897/zookeys.1087.76720. PMC 8897373 Check
|pmc=value (help). PMID 35585942 Check|pmid=value (help).
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