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Transmembrane protein 236

From EverybodyWiki Bios & Wiki



Transmembrane protein 236 (TMEM236) is a protein that, in humans, is encoded by the transmembrane protein 236 gene.[1] TMEM128 contains 6 transmembrane domains and 1 disordered region.

Gene

The TMEM236 gene is located at 10p12.33.[2] The TMEM236 gene contains 4 exons with a length of 5515 nucleotides.[2] TMEM236 is also known by the aliases: family with sequence similarity 23, member A (FAM23A) and family with sequence similarity 23, member B (FAM23B).[1]

Transcripts

There is one known isoform of TMEM236, transmembrane protein 236 isoform X1.[1] TMEM236 isoform X1 is 289 aa [3] and is missing exon 1 of TMEM236. [1]

Protein

Transmembrane Protein 236 or TMEM236, is predicted to have a molecular weight of 39.7 kDa [4] and isoelectric point (pI) of 9.36.[5]. TMEM236 is 351 aa long and contains 6 experimentally confirmed transmembrane domains along with 1 experimentally confirmed disordered region.[2]

Structure

The secondary structure of TMEM236 contains predicted Alpha Helix regions (25.07%), Extended Strand regions (26.21%), and Random Coil regions (48.71%).[6]

File:TMEM236 Secondary structure.png
Secondary structure of TMEM236. Blue indicates probable alpha helix, red indicates the predicted extended strand, and purple indicates a random coil.

The AlphaFold predicted tertiary structure of the protein shows many similarities to the predicted secondary structure.[7]

File:TMEM236 AlphaFold Predicted Structure.png
Tertiary structure of TMEM236 as predicted by AlphaFold. The N-terminus is shown in red and the C-terminus is shown in purple.

Gene level regulation

TMEM236 is most highly expressed in the small intestine, duodenum, and colon; it is also expressed in the appendix and placenta at a lesser rate. [1]

Protein level regulation

There is an experimentally confirmed N-Glycosylation site at 193-196[2]; along with predicted Casein kinase II phosphorylation, N-myristoylation, Protein kinase C phosphorylation, and Tyrosine kinase phosphorylation sites.[8]

-
Modification Location
N-Glycosylation 193-196
Casein kinase II phosphorylation 174-177

229-232
344-347

N-Myristoylation 154-159

307-312
320-32

Protein kinase C phosphorylation 3-5

155-157
229-231

Tyrosine kinase phosphorylation 39-47

Homology

There are no paralogs found for TMEM236. TMEM236 was found to be conserved in fish, but not found in any reptilia, amphibia, or aves on record.[9]

File:Ortholog Table for TMEM236.png
Ortholog table for TMEM236, ordered by their median date of divergence from Humans. Mammals are in red, Marsupials in purple, and Fish in blue.

Interacting Proteins

TMEM236 is suspected to interact with 10 different proteins, this information was found using the STRING program, each interaction was found via text mining.[10]

Interactions of TMEM236
Gene Full name Location
LRRC14B Leucine-rich repeat-containing protein 14B chr5:191,495-196,334
OR13C8 Olfactory receptor 13C8 chr9:104,569,168-104,570,130
IQSEC3 IQ motif and SEC7 domain-containing protein 3 chr12:66,767-178,455
TMEM191B Transmembrane protein 191B chr22:18,527,802-18,530,573
CALN1 Calcium-binding protein 8 chr7:71,779,491-72,504,279
TTC9 Tetratricopeptide repeat protein 9a chr14:70,641,916-70,675,366
MYCBP2 E3 ubiquitin-protein ligase MYCBP2 chr13:77,042,474-77,327,094
RBMS3 RNA-binding motif, single-stranded-

interacting protein 3

chr3:28,574,791-30,010,395
MYO16 Unconventional myosin-XVI chr13:108,495,716-109,208,005
C1orf115 Chromosome 1 open reading frame 115 chr1:220,690,363-220,699,153

References

  1. 1.0 1.1 1.2 1.3 1.4 "TMEM236 transmembrane protein 236 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov.
  2. 2.0 2.1 2.2 2.3 "Homo sapiens transmembrane protein 236 (TMEM236), mRNA". April 2, 2023 – via NCBI Nucleotide.
  3. "transmembrane protein 236 isoform X1 [Homo sapiens] - Protein - NCBI". www.ncbi.nlm.nih.gov.
  4. "SAPS Results". www.ebi.ac.uk.
  5. "SIB Swiss Institute of Bioinformatics | Expasy". www.expasy.org.
  6. "[unknown]". Network Protein Sequence @nalysis.
  7. "AlphaFold Protein Structure Database". alphafold.ebi.ac.uk.
  8. "Motif Scan". myhits.sib.swiss.
  9. "BLAST: Basic Local Alignment Search Tool". blast.ncbi.nlm.nih.gov.
  10. "[unknown]". STRING CONSORTIUM.


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