CLīM ™ Technology
Script error: No such module "AfC submission catcheck".
Protein purification is a primary step and the basis for numerous biochemical and biomedical studies. It is particularly crucial for high-resolution structural analysis and industrial protein production, where it has to meet the high-yield, high-purity, and high-activity (HHH) requirement. However, the HHH purification of many proteins or protein complexes remains a difficult, target- dependent, and multistep process. The CLiM Affinity Tag System allows for one-step HHH purification of a wide range of traditionally challenging biological molecules, including eukaryotic, membrane, toxic, and DNA/RNA-binding proteins and complexes. CLiM Affinity Tag System overcomes the limitations of existing chromatography systems and provides several crucial advantages. It may emerge as the most efficient and potentially universal tool for high-throughput studies of many significant biological systems. intermolecular interactions.
CL7 Tag
The CL7 tag can be expressed at the N or C terminus of the target protein. Our plasmids offer combinations of solubility tags, affinity tags, and cleavage sites with multiple cloning options. CL7 showed no negative effect on solubility or expression in control experiments. In fact, CL7 improves expression levels and solubility of proteins originally insoluble when expressed in E. coli with no tag. Several clinically and therapeutically relevant proteins moved from the insoluble to the soluble fraction when expressed with a CL7 tag. No denaturants or refolding from inclusion bodies was required to purify the solubly-expressed proteins. These proteins demonstrated biological activities equivalent to that of other commercial/clinical samples in cell-
based assays.
Im7 Resin
lm7 resin is composed of CL7';s binding partner, lm7, immobilized to agarose resin. The highly specific affinity between lm7 and CL7 results in minimal off-target resin binding. The high resin binding capacity of 35-40 mg/mL allows for large amounts of CL7-tagged protein to be captured. The lm7 domain is highly resilient: the resin can be regenerated and reused up to 100 times using Gdn-HCI.
References[edit]
This article "CLīM ™ Technology" is from Wikipedia. The list of its authors can be seen in its historical and/or the page Edithistory:CLīM ™ Technology. Articles copied from Draft Namespace on Wikipedia could be seen on the Draft Namespace of Wikipedia and not main one.
