Garrett A. Perchetti
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Garrett A. Perchetti is an American research scientist at the University of Washington School of Medicine, Virology Division in Seattle, WA. Perchetti's research focuses on respiratory viruses and methods to broaden access to COVID-19 testing[1][2][3]. He is an editor with the Journal of Medical Virology and does research with Next Generation Sequencing, Virology, and Infectious Diseases. [4]. Biotech companies like Sigma-Aldrich and Zymo Research have also utilized Perchetti's work on COVID-19 specimen pooling and assay performance to develop commercial laboratory diagnostic products[5][6]. In particular, his research regarding rapid antigen test sensitivity[7][8], especially on new COVID-19 variants,[9][10] has been critical to opening up schools and characterizes the limitations and utility of widespread, decentralized, rapid testing[11]. He also described the first instance of SARS-CoV-2 variants affecting rapid antigen test performance[12]
History[edit]
During global supply chain shortages in 2020, Perchetti demonstrated that transport and storage of SARS-CoV-2 in a much more available and affordable alternative (phosphate-buffered saline) was comparable to viral transport medium, relieving pressure on high-demand vital reagents involved in diagnostic testing[13]. In collaboration with Trevor Bedford[14], Helen Y. Chu[15], Keith R. Jerome[16], and Alexander L. Greninger [17][18], Perchetti detected the first instance of a COVID variant of concern in Washington State,( B.1.1.7-alpha) originally from the U.K.[19][20]. In efforts to address the global bottleneck of diagnostic reagents, Perchetti and colleagues designed an extraction-free method for detecting SARS-CoV-2 that dramatically reduced crucial reagent use, assay cost, and sample-to-answer turn around time[21][22]. The assay gain significant attention on the Cold Spring Harbor preprint server as one of its most downloaded publications, ranking in the top 0.08% of all published research outlets[23].
His research on Herpes Simplex Virus (HSV) has demonstrated for the first time that two species of HSV (oral and genital) can recombine to form new strains to increase the virus's genetic diversity[24][25]. Recently, his work has described SARS-CoV-2 infectivity demonstrating no culturable virus is detected beyond an RT-PCR cycle threshold of 31[26]. In collaboration with the Fred Hutchinson Cancer Research Institute, Perchetti reported on the incidence of viral drug resistance for the novel therapeutic Letermovir, designed and produced by Merck to treat CMV prophylactically in transplant patients[27]. In 2020, he teamed up with researchers from Johns Hopkins, the Milken Institute, and Seattle Children's Hospital and sequenced the first complete HMPV glycoproteins from Nepalese infants in efforts to increase the diversity of publicly available sequences of respiratory viruses[28]. Reuters featured Perchetti as a frontline healthcare worker during the COVID pandemic and in 2021 was nominated for Forbes 30 under 30[29]. Perchetti serves on microbiology editorial boards, is a referee in multiple medical journals for viral research, and is a member of AAAS, AMA, ASM, ACP and ASV. He attended Harvard University and the University of Washington receiving a BA (Art, Art History, and Design) and a BS (Microbiology),[30] and sits on the Eastlake Community Council in Seattle, WA[31]
Selected publications[edit]
- Nalla AK, Casto AM, Huang M-LW, Perchetti GA, Sampoleo R, Shrestha L, Wei Y, Zhu H, Greninger AL, Jerome KR. Comparative Performance of SARS-CoV-2 Detection Assays using Seven Different Primer/Probe Sets and One Assay Kit. Journal of Clinical Microbiology. 2020 Apr 8; JCM.00557-20, doi: 10.1128/JCM.00557-20
- Perchetti GA, Nalla AK, Huang ML, Zhu H, Wei Y, Stensland L, Loprieno M, Jerome KR, Greninger AL. Validation of SARS-CoV-2 detection across multiple specimen types. Journal of Clinical Virology. May 12, 2020. 104438, doi: 10.1016/j.jcv.2020.
- Perchetti GA, Huang ML, Peddu V, Jerome KR, Greninger AL. Stability of SARS-CoV-2 RNA in Phosphate-Buffered Saline. Journal of Clinical Microbiology. May 15, 2020. 58; e01094-20. doi: 10.1128/JCM.01094-20
- Perchetti GA, Nalla AK, Huang ML, Zhu H, Wei Y, Jerome KR, Greninger AL. Multiplexing qRT-PCR assay for SARS-CoV-2. Journal of Clinical Virology. June 8, 2020. 129; 104499, doi: 10.1016/j.jcv.2020.104499
- Bruce EA, Huang ML, Perchetti GA, Tighe S, Hoffman JJ, Laaguiby P, Gerrard DL, Nalla A, Wei Y, Greninger AL, Diehl SA, Shirley DJ, Leonard DGB, Huston CD, Kirkpatrick BD, Dragon J, Crothers JW, Jerome KR, Botten JW. Direct RT-qPCR Detection of SARS-CoV-2 RNA From Patient Nasopharyngeal Swabs Without an RNA Extraction Step. Plos Biology. October 2, 2020. doi: 10.1101/2020.03.20.001008
- Perchetti GA, Huang ML, Mills MG, Jerome KR, Greninger AL. Analytical Sensitivity of the Abbott BinaxNOW COVID-19 Ag CARD. Journal of Clinical Microbiology. December 11, 2020. doi: 10.1128/JCM.02880-20
- Perchetti GA, Pepper G, Shrestha L, LaTurner K, Kim DY, Huang ML, Jerome KR, Greninger AL. Performance Characteristic of the Abbott Alinity m SARS-CoV-2 Assay. Journal of Clinical Virology. May 14, 2021. doi: 10.1016/j.jcv.2021.104869
- Bourassa L*, Perchetti GA*, Phung Q, Lin MJ, Mills MG, Roychoudhury P, Harmon KG, Reed JC, Greninger AL. A SARS-CoV-2 Nucleocapsid Variant that Affects Antigen Test Performance. Journal of Clinical Virology. June 17, 2021. doi: 10.1016/j.jcv.2021.104900
- Perchetti GA, Zhu H, Mills MG, Shrestha L, Wagner C, Bakhash SM, Lin M, Xie H, Huang M-L, Mathias P, Bedford T, Jerome KR, Greninger AL, Roychoudhury P. Specific allelic discrimination of N501Y and other SARS-CoV-2 mutations by ddPCR detects B.1.1.7 lineage in Washington State. Journal of Medical Virology. June 25, 2021. doi: 10.1002/jmv.27155
- Bruce EA, Mills MG, Sampoleo R, Perchetti GA, Huang ML, Despres HW, Schmidt MM, Roychoudhury P, Shirley DJ, Jerome KR, Greninger AL, Botten JW. Predicting Infectivity: Comparing Four PCR-based Assays to Detect Culturable SARS-CoV-2 in Clinical Samples. December 3, 2021. EMBO Molecular Medicine. E15290. doi: 10.15252/emmm.202115290
References[edit]
- ↑ Perchetti, G. A.; Nalla, A. K.; Huang, M. L.; Jerome, K. R.; Greninger, A. L. (2020). "Multiplexing primer/Probe sets for detection of SARS-CoV-2 by QRT-PCR". Journal of Clinical Virology : The Official Publication of the Pan American Society for Clinical Virology. 129: 104499. doi:10.1016/j.jcv.2020.104499. PMC 7278635 Check
|pmc=value (help). PMID 32535397 Check|pmid=value (help). - ↑ Perchetti, G. A.; Sullivan, K. W.; Pepper, G.; Huang, M. L.; Breit, N.; Mathias, P.; Jerome, K. R.; Greninger, A. L. (2020). "Pooling of SARS-CoV-2 samples to increase molecular testing throughput". Journal of Clinical Virology : The Official Publication of the Pan American Society for Clinical Virology. 131: 104570. doi:10.1016/j.jcv.2020.104570. PMC 7396208 Check
|pmc=value (help). PMID 32805524 Check|pmid=value (help). - ↑ Bruce, Emily A.; Huang, Meei-Li; Perchetti, Garrett A.; Tighe, Scott; Laaguiby, Pheobe; Hoffman, Jessica J.; Gerrard, Diana L.; Nalla, Arun K.; Wei, Yulun; Greninger, Alexander L.; Diehl, Sean A.; Shirley, David J.; Leonard, Debra G. B.; Huston, Christopher D.; Kirkpatrick, Beth D.; Dragon, Julie A.; Crothers, Jessica W.; Jerome, Keith R.; Botten, Jason W. (2020). "Direct RT-QPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step". PLOS Biology. 18 (10): e3000896. doi:10.1371/journal.pbio.3000896. PMC 7556528 Check
|pmc=value (help). PMID 33006983 Check|pmid=value (help). - ↑ "Journal of Medical Virology Editor Biographies".
- ↑ https://www.sigmaaldrich.com/BB/en/search/100-174?focus=papers&page=1&perpage=30&sort=relevance&term=100%20174&type=citation_search
- ↑ "The Importance of Sample Pooling in COVID-19 Testing".
- ↑ "Rapid antigen test is far less sensitive than lab standard". newsroom.uw.edu. 2020-12-16. Retrieved 2022-03-30.
- ↑ Perchetti, Garrett A.; Huang, Meei-Li; Mills, Margaret G.; Jerome, Keith R.; Greninger, Alexander L. (2021). "Analytical Sensitivity of the Abbott BinaxNOW COVID-19 Ag Card". Journal of Clinical Microbiology. 59 (3). doi:10.1128/JCM.02880-20. PMC 8106729 Check
|pmc=value (help). PMID 33310764 Check|pmid=value (help). - ↑ Bourassa, Lori; Perchetti, Garrett A.; Phung, Quynh; Lin, Michelle J.; Mills, Margaret G.; Roychoudhury, Pavitra; Harmon, Kimberly G.; Reed, Jonathan C.; Greninger, Alexander L. (2021). "A SARS-CoV-2 Nucleocapsid Variant that Affects Antigen Test Performance". Journal of Clinical Virology. 141: 104900. doi:10.1016/j.jcv.2021.104900. PMC 8219478 Check
|pmc=value (help). PMID 34171548 Check|pmid=value (help). - ↑ "Specific Allelic Discrimination of N501Y and Other SARS-CoV-2 Mutations by DDPCR Detects B.1.1.7 Lineage in Washington State".
- ↑ News, Mirage (2020-12-16). "Study ID's limited value of rapid COVID-19 antigen test". Mirage News. Retrieved 2022-03-30.
- ↑ "Lab reports 1st instance of SARS-CoV-2 variants affecting antigen test performance". 10 May 2021.
- ↑ Perchetti, Garrett A.; Huang, Meei-Li; Peddu, Vikas; Jerome, Keith R.; Greninger, Alexander L. (2020). "Stability of SARS-CoV-2 in Phosphate-Buffered Saline for Molecular Detection". Journal of Clinical Microbiology. 58 (8). doi:10.1128/JCM.01094-20. PMC 7383534 Check
|pmc=value (help). PMID 32414839 Check|pmid=value (help). - ↑ "Two Seattleites, an infectious disease scientist and a poet, win MacArthur 'genius grants'". 28 September 2021.
- ↑ Fink, Sheri (11 March 2020). "'It's Just Everywhere Already': How Delays in Testing Set Back the U.S. Coronavirus Response". The New York Times.
- ↑ "Keith Jerome, M.D., Ph.D."
- ↑ "Innovator of the Year awarded to directors of virology lab". 28 April 2021.
- ↑ Jewett, Christina (3 January 2022). "With no way to identify Omicron and Delta patients, treatment decisions are vexing doctors". The New York Times.
- ↑ Perchetti, Garrett A.; Zhu, Haiying; Mills, Margaret G.; Shrestha, Lasata; Wagner, Cassia; Bakhash, Shah Mohamed; Lin, Michelle J.; Xie, Hong; Huang, Meei‐Li; Mathias, Patrick; Bedford, Trevor; Jerome, Keith R.; Greninger, Alexander L.; Roychoudhury, Pavitra (2021). "Specific allelic discrimination of N501Y and other SARS‐CoV‐2 mutations by DDPCR detects B.1.1.7 lineage in Washington State". Journal of Medical Virology. 93 (10): 5931–5941. doi:10.1002/jmv.27155. PMC 8427099 Check
|pmc=value (help). PMID 34170525 Check|pmid=value (help). - ↑ "Specific allelic discrimination of N501Y and other SARS-CoV-2 mutations by DDPCR detects B.1.1.7 lineage in Washington State".
- ↑ "New Covid-19 test doesn't need reagents that are in short supply".
- ↑ "Extraction-free and Saliva COVID-19 Testing". 11 May 2020.
- ↑ "Altmetric – DIRECT RT-QPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP".
- ↑ "The Oral and Genital Herpes Viruses Are Having 'Sex.' the Result is Worrisome". Live Science. 3 May 2019.
- ↑ "Line is blurring between human herpes simplex viruses". 26 April 2019.
- ↑ Bruce, Emily A.; Mills, Margaret G.; Sampoleo, Reigran; Perchetti, Garrett A.; Huang, Meei‐Li; Despres, Hannah W.; Schmidt, Madaline M.; Roychoudhury, Pavitra; Shirley, David J.; Jerome, Keith R.; Greninger, Alexander L.; Botten, Jason W. (2022). "Predicting infectivity: Comparing four PCR‐based assays to detect culturable SARS‐CoV‐2 in clinical samples". EMBO Molecular Medicine. 14 (2): e15290. doi:10.15252/emmm.202115290. PMC 8819313 Check
|pmc=value (help). PMID 34862752 Check|pmid=value (help). - ↑ Zamora, Danniel; Perchetti, Garrett; Biernacki, Melinda; Xie, Hu; Castor, Jared L; Joncas-schronce, Laurel; Blazevic, Rachel; Leisenring, Wendy; Huang, Meei-Li; Jerome, Keith; Martin, Paul J (2021-12-04). "20. Risk Factors for Breakthrough Cytomegalovirus (CMV) Infection and De Novo Resistance in Hematopoietic Cell Transplantation (HCT) Recipients Receiving Letermovir Prophylaxis". Open Forum Infectious Diseases. 8 (Supplement_1): S13–S14. doi:10.1093/ofid/ofab466.020. ISSN 2328-8957. PMC 8643935 Check
|pmc=value (help). - ↑ Perchetti, Garrett A; Wilcox, Naomi; Chu, Helen Y; Katz, Joanne; Khatry, Subarna K; LeClerq, Steven C; Tielsch, James M; Jerome, Keith R; Englund, Janet A; Kuypers, Jane (2021-04-30). "Human Metapneumovirus Infection and Genotyping of Infants in Rural Nepal". Journal of the Pediatric Infectious Diseases Society. 10 (4): 408–416. doi:10.1093/jpids/piaa118. ISSN 2048-7207. PMID 33137178 Check
|pmid=value (help). - ↑ https://www.reuters.com/news/picture/seattle-hospital-staff-reflect-on-their-idUSRTX7DN3P
- ↑ "Where Art and Science Meet | the Huddle". 7 November 2019.
- ↑ "The Eastlake News" (PDF). 2020.
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