📜 Post with us on Reddit
📝 Create a new article
🏭 Create a company page
🇬🇧 - in English
🇫🇷 - en Français (FR)
🇵🇹 - em Português (PT)
🇩🇪 - auf Deutsch (DE)
🇯🇵 - 日本語で (JA)
Sponsored articles

You can edit almost every page by Creating an account and confirming your email.

Gerardo Turcatti

From EverybodyWiki Bios & Wiki


Professor
Gerardo Turcatti
EPFL 2020 Gerardo Turcatti Portrait.jpg EPFL 2020 Gerardo Turcatti Portrait.jpg
Gerardo Turcatti in 2020
Born1959 (age 66–67)
Montevideo, Uruguay
💼 Occupation
Known forHigh-throughput screening
TitleAdjunct Professor
🌐 Websitehttps://www.epfl.ch/research/facilities/biomolecular-screening/

Gerardo Turcatti (born 1959 in Montevideo, Uruguay) is a Swiss-Uruguayan chemical biologist specialized in chemical biology and drug development. He is a professor at the EPFL (École Polytechnique Fédérale de Lausanne) and head of EPFL's Biomolecular Screening Facility at the School of Life Sciences.[1][2][3]

Early life

Turcatti was born and raised in Uruguay in a family of seven children. From 17 years onward, he was mostly earning his living and his studies on his own.

Career

Turcatti studied chemistry at the University of the Republic (Uruguay) up to the Bachelor level. He then moved to Switzerland to earn a Master's degree in chemical engineering from the University of Geneva. Discovering the world of life sciences while working at the biotech company Biogen during his bachelor and master studies, he dropped his initial plan to become an chemical engineer. Ever since he has worked as a chemical biologist. He joined Horst Vogel at EPFL and the Glaxo Biomedical Research Institute in Geneva for a PhD thesis in chemistry and biochemistry on "Novel fluorescence-based approaches to probe ligand recognition and structure of the tachykinin NK2 receptor". [4][5][6][7] His thesis was awarded as best thesis with the EPFL award in 1995.[8]

Until 1997 he was a research scientist at the Biochemistry Department of the Glaxo Wellcome Biomedical Research Institute in Geneva, where he further adapted applications of the methodologies he developed during his PhD to other G protein-coupled receptor (GPCR) pharmacological relevant targets such as receptors GPCR (neurokinins, chemokines, vasopressins) that enabled the design and characterization of ligands with applicability in drug discovery.[9][10][11][12] In 1998, he went to work as a senior scientist at the Genomic Technologies Department of the Serono Pharmaceutical Research Institute.[13]

In January 2001, he founded Manteia Predictive Medicine and became executive technology director. The Swiss-based company developed novel high throughput DNA sequencing technologies and is currently owned by Illumina.[14] Starting in 2004, he acted as an independent consultant for companies from the life science sector such as Lynx Therapeutics, Solexa Ltd, SpinX Technologies and Serono.[15]

In 2006, he founded the Biomolecular Screening Facility (BSF) at EPFL and has been its director since its inception.[16][17] In 2020, Turcatti became Adjunct Professor at EPFL.[1] In the framework of the NCCR research project on chemical biology, he is the project leader of the academic platform for chemical screens (ACCESS project).[18]

Research

The research of the Turcatti's laboratory focuses in methods and technologies for efficient early drug discovery processes enabling more specific and safer molecules as therapeutic agents, such as high throughput screening. The laboratory also engages directly in drug development.

Their research has lead to higher throughput rates in DNA sequencing. They developed among others new surface molecules for DNA attachment for DNA templates amplification[19][20]; massive parallel amplification of DNA templates;[21][22][23] a DNA sequencing analysis using chemically engineered fluorescent nucleotides transient terminators;[24] a fluorescent microscopy setup for high throughput enabling low cost whole genome analysis;[17][16] and a fully automatized microfluidic system.[25] Most of the DNA-related technologies they developed currently used in commercial instrument produced by Illumina.

Their research also targets early drug discovery via the development of efficient methods for drug screening such as highly informative assays.[26] By developing methods to estimate efficacy, potency and early detection of off-target effects of potential drugs, they aim at the acceleration of drug development.[27][28][29][30]

Distinctions

Turcatti is a member of the scientific advisory board of ChemBioFrance,[31] the scientific advisory board of Innopharma,[32] and the European Cellular Assays Interest Group (EuCAI), [33] and the editorial board of the journal Combinatorial Chemistry and High Throughput Screening.[34]

Selected works

  • Georgi, Fanny; Kuttler, Fabien; Murer, Luca; Andriasyan, Vardan; Witte, Robert; Yakimovich, Artur; Turcatti, Gerardo; Greber, Urs F. (2020). "A high-content image-based drug screen of clinical compounds against cell transmission of adenovirus". Scientific Data. 7 (1): 265. Bibcode:2020NatSD...7..265G. doi:10.1038/s41597-020-00604-0. PMC 7423605 Check |pmc= value (help). PMID 32788590 Check |pmid= value (help).
  • Georgi, Fanny; Andriasyan, Vardan; Witte, Robert; Murer, Luca; Hemmi, Silvio; Yu, Lisa; Grove, Melanie; Meili, Nicole; Kuttler, Fabien; Yakimovich, Artur; Turcatti, Gerardo; Greber, Urs F. (2020). "The FDA-Approved Drug Nelfinavir Inhibits Lytic Cell-Free but Not Cell-Associated Nonlytic Transmission of Human Adenovirus". Antimicrobial Agents and Chemotherapy. 64 (9). doi:10.1128/AAC.01002-20. PMC 7449217 Check |pmc= value (help). PMID 32601166 Check |pmid= value (help).
  • Brandenberg, Nathalie; Hoehnel, Sylke; Kuttler, Fabien; Homicsko, Krisztian; Ceroni, Camilla; Ringel, Till; Gjorevski, Nikolce; Schwank, Gerald; Coukos, George; Turcatti, Gerardo; Lutolf, Matthias P. (2020). "High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays". Nature Biomedical Engineering. 4 (9): 863–874. doi:10.1038/s41551-020-0565-2. PMID 32514094 Check |pmid= value (help). Unknown parameter |s2cid= ignored (help)
  • Jaferzadeh, Keyvan; Rappaz, Benjamin; Kuttler, Fabien; Kim, Bo Kyoung; Moon, Inkyu; Marquet, Pierre; Turcatti, Gerardo (2020). "Marker-Free Automatic Quantification of Drug-Treated Cardiomyocytes with Digital Holographic Imaging". ACS Photonics. 7: 105–113. doi:10.1021/acsphotonics.9b01152.
  • Rappaz, Benjamin; Jourdain, Pascal; Banfi, Damiano; Kuttler, Fabien; Marquet, Pierre; Turcatti, Gerardo (2020). "Image-Based Marker-Free Screening of GABAA Agonists, Antagonists, and Modulators". Slas Discovery: Advancing the Science of Drug Discovery. 25 (5): 458–470. doi:10.1177/2472555219887142. PMC 7243081 Check |pmc= value (help). PMID 31779505.
  • Haag, Simone M.; Gulen, Muhammet F.; Reymond, Luc; Gibelin, Antoine; Abrami, Laurence; Decout, Alexiane; Heymann, Michael; Van Der Goot, F. Gisou; Turcatti, Gerardo; Behrendt, Rayk; Ablasser, Andrea (2018). "Targeting STING with covalent small-molecule inhibitors". Nature. 559 (7713): 269–273. Bibcode:2018Natur.559..269H. doi:10.1038/s41586-018-0287-8. PMID 29973723. Unknown parameter |s2cid= ignored (help)
  • Horvath, Peter; Aulner, Nathalie; Bickle, Marc; Davies, Anthony M.; Nery, Elaine Del; Ebner, Daniel; Montoya, Maria C.; Östling, Päivi; Pietiäinen, Vilja; Price, Leo S.; Shorte, Spencer L.; Turcatti, Gerardo; von Schantz, Carina; Carragher, Neil O. (2016). "Screening out irrelevant cell-based models of disease" (PDF). Nature Reviews Drug Discovery. 15 (11): 751–769. doi:10.1038/nrd.2016.175. PMID 27616293. Unknown parameter |s2cid= ignored (help)
  • Rappaz, Benjamin; Kuttler, Fabien; Breton, Billy; Turcatti, Gerardo (2015). "Digital Holographic Imaging for Label-Free Phenotypic Profiling, Cytotoxicity, and Chloride Channels Target Screening". Label-Free Biosensor Methods in Drug Discovery. Methods in Pharmacology and Toxicology. pp. 307–325. doi:10.1007/978-1-4939-2617-6_17. ISBN 978-1-4939-2616-9. Search this book on

References

  1. ↑ 1.0 1.1 "13 new professors appointed at ETH Zurich and EPFL | ETH-Board". www.ethrat.ch. Archived from the original on 2020-10-30. Retrieved 2020-12-28.
  2. ↑ "Biomolecular Screening Facility". www.epfl.ch. Retrieved 2020-12-28.
  3. ↑ "Gerardo Turcatti". people.epfl.ch. Retrieved 2020-12-28.
  4. ↑ Turcatti, Gerardo (1995). "Novel fluorescence - based approaches to probe ligand recognition and structure of the tachykinin NK2 receptor". Infoscience. doi:10.5075/epfl-thesis-1426.
  5. ↑ Turcatti, Gerardo; Vogel, Horst; Chollet, Andre (1995-03-28). "Probing the Binding Domain of the NK2 Receptor with Fluorescent Ligands: Evidence That Heptapeptide Agonists and Antagonists Bind Differently". Biochemistry. 34 (12): 3972–3980. doi:10.1021/bi00012a015. ISSN 0006-2960. PMID 7696262.
  6. ↑ Turcatti, Gerardo; Nemeth, Karin; Edgerton, Michael D.; Meseth, Ulrich; Talabot, François; Peitsch, Manuel; Knowles, Jonathan; Vogel, Horst; Chollet, André (1996-08-16). "Probing the Structure and Function of the Tachykinin Neurokinin-2 Receptor through Biosynthetic Incorporation of Fluorescent Amino Acids at Specific Sites". Journal of Biological Chemistry. 271 (33): 19991–19998. doi:10.1074/jbc.271.33.19991. ISSN 0021-9258. PMID 8702716. Unknown parameter |s2cid= ignored (help)
  7. ↑ "Gerardo Turcatti". people.epfl.ch. Retrieved 2020-12-30.
  8. ↑ "EPFL Research Prizes past laureates - Auto EPFL Doctorate award.pdf". Google Docs. Retrieved 2020-12-28.
  9. ↑ Mukhopadhyay, P.; Huber, T.; Sakmar, T.P. (2011), "Chapter 19. Probing the Activation Mechanism of Heptahelical Receptors: Experimental Validation of Molecular Dynamics Simulations", Drug Discovery, Cambridge: Royal Society of Chemistry, pp. 375–383, doi:10.1039/9781849733441-00375, ISBN 978-1-84973-183-6, retrieved 2020-12-30
  10. ↑ Chollet, Andre; Turcatti, Gerardo (2010-06-12). "Biosynthetic Incorporation of Unnatural Amino Acids into G Protein-Coupled Receptors". ChemInform. 30 (51): no. doi:10.1002/chin.199951288. ISSN 0931-7597.
  11. ↑ Chollet, André; Turcatti, Gerardo (1999). "Biophysical approaches to G protein-coupled receptors: Structure, function and dynamics". Journal of Computer-Aided Molecular Design. 13 (3): 209–219. Bibcode:1999JCAMD..13..209C. doi:10.1023/a:1008052002695. ISSN 0920-654X. PMID 10216829. Unknown parameter |s2cid= ignored (help)
  12. ↑ Chollet, André; Turcatti, Gerardo (May 1998). "Mapping the binding sites of peptide and non-peptide molecules to G protein-coupled receptors by fluorescence". Letters in Peptide Science. 5 (2–3): 79–82. doi:10.1007/bf02443443. ISSN 0929-5666. Unknown parameter |s2cid= ignored (help)
  13. ↑ Adessi, C. (2000-10-15). "Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms". Nucleic Acids Research. 28 (20): 87e–87. doi:10.1093/nar/28.20.e87. ISSN 1362-4962. PMC 110803. PMID 11024189.
  14. ↑ Dodge, Arash; Turcatti, Gerardo; Lawrence, Isabelle; de Rooij, Nico F.; Verpoorte, Elisabeth (March 2004). "A Microfluidic Platform Using Molecular Beacon-Based Temperature Calibration for Thermal Dehybridization of Surface-Bound DNA". Analytical Chemistry. 76 (6): 1778–1787. doi:10.1021/ac034377+. ISSN 0003-2700. PMID 15018583.
  15. ↑ Fedurco, M. (2006-02-06). "BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies". Nucleic Acids Research. 34 (3): e22. doi:10.1093/nar/gnj023. ISSN 0305-1048. PMC 1363783. PMID 16473845.
  16. ↑ 16.0 16.1 Bentley, David R.; Balasubramanian, Shankar; Swerdlow, Harold P.; Smith, Geoffrey P.; Milton, John; Brown, Clive G.; Hall, Kevin P.; Evers, Dirk J.; Barnes, Colin L.; Bignell, Helen R.; Boutell, Jonathan M. (November 2008). "Accurate whole human genome sequencing using reversible terminator chemistry". Nature. 456 (7218): 53–59. Bibcode:2008Natur.456...53B. doi:10.1038/nature07517. ISSN 1476-4687. PMC 2581791. PMID 18987734.
  17. ↑ 17.0 17.1 Turcatti, Gerardo; Romieu, Anthony; Fedurco, Milan; Tairi, Ana-Paula (2008-02-07). "A new class of cleavable fluorescent nucleotides: synthesis and optimization as reversible terminators for DNA sequencing by synthesis". Nucleic Acids Research. 36 (4): e25. doi:10.1093/nar/gkn021. ISSN 1362-4962. PMC 2275100. PMID 18263613.
  18. ↑ "Gerardo Turcatti, Head of BSF-ACCESS - NCCR Chemical Biology". NCCR in Chemical Biology. Retrieved 2021-01-05.
  19. ↑ Colombo, Gualtiero; Gatti, Stefano; Turcatti, Flavia; Sordi, Andrea; Fassati, Luigi R.; Bonino, Ferruccio; Lipton, James M.; Catania, Anna (2005-08-22). "Gene Expression Profiling Reveals Multiple Protective Influences of the Peptide α-Melanocyte-Stimulating Hormone in Experimental Heart Transplantation". The Journal of Immunology. 175 (5): 3391–3401. doi:10.4049/jimmunol.175.5.3391. ISSN 0022-1767. PMID 16116233. Unknown parameter |s2cid= ignored (help)
  20. ↑ Fedurco, M. (2006-02-06). "BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies". Nucleic Acids Research. 34 (3): e22. doi:10.1093/nar/gnj023. ISSN 0305-1048. PMC 1363783. PMID 16473845.
  21. ↑ [1], "Methods of nucleic acid amplification and sequencing", issued 2014-01-08 
  22. ↑ [2], "Method of nucleotide detection", issued 2014-06-03 
  23. ↑ [3], "Preparation of templates for nucleic acid sequencing", issued 2006-07-20 
  24. ↑ Turcatti, Gerardo; Romieu, Anthony; Fedurco, Milan; Tairi, Ana-Paula (2008-02-07). "A new class of cleavable fluorescent nucleotides: synthesis and optimization as reversible terminators for DNA sequencing by synthesis †". Nucleic Acids Research. 36 (4): e25. doi:10.1093/nar/gkn021. ISSN 1362-4962. PMC 2275100. PMID 18263613.
  25. ↑ Dodge, Arash; Turcatti, Gerardo; Lawrence, Isabelle; de Rooij, Nico F.; Verpoorte, Elisabeth (2004-03-01). "A Microfluidic Platform Using Molecular Beacon-Based Temperature Calibration for Thermal Dehybridization of Surface-Bound DNA". Analytical Chemistry. 76 (6): 1778–1787. doi:10.1021/ac034377+. ISSN 0003-2700. PMID 15018583.
  26. ↑ Horvath, Peter; Aulner, Nathalie; Bickle, Marc; Davies, Anthony M.; Nery, Elaine Del; Ebner, Daniel; Montoya, Maria C.; Östling, Päivi; Pietiäinen, Vilja; Price, Leo S.; Shorte, Spencer L. (November 2016). "Screening out irrelevant cell-based models of disease". Nature Reviews Drug Discovery. 15 (11): 751–769. doi:10.1038/nrd.2016.175. ISSN 1474-1784. PMID 27616293. Unknown parameter |s2cid= ignored (help)
  27. ↑ Jaferzadeh, Keyvan; Rappaz, Benjamin; Kuttler, Fabien; Kim, Bo kyoung; Moon, Inkyu; Marquet, Pierre; Turcatti, Gerardo (2020-01-15). "Marker-Free Automatic Quantification of Drug-Treated Cardiomyocytes with Digital Holographic Imaging". ACS Photonics. 7 (1): 105–113. doi:10.1021/acsphotonics.9b01152.
  28. ↑ Rappaz, Benjamin; Jourdain, Pascal; Banfi, Damiano; Kuttler, Fabien; Marquet, Pierre; Turcatti, Gerardo (2019-11-28). "Image-Based Marker-Free Screening of GABAA Agonists, Antagonists, and Modulators". SLAS DISCOVERY: Advancing the Science of Drug Discovery. 25 (5): 458–470. doi:10.1177/2472555219887142. PMC 7243081 Check |pmc= value (help). PMID 31779505.
  29. ↑ Campos, Vasco; Rappaz, Benjamin; Kuttler, Fabien; Turcatti, Gerardo; Naveiras, Olaia (2018-04-05). "High-throughput, nonperturbing quantification of lipid droplets with digital holographic microscopy". Journal of Lipid Research. 59 (7): 1301–1310. doi:10.1194/jlr.d085217. ISSN 0022-2275. PMC 6027917. PMID 29622579.
  30. ↑ Jaferzadeh, Keyvan; Moon, Inkyu; Bardyn, Manon; Prudent, Michel; Tissot, Jean-Daniel; Rappaz, Benjamin; Javidi, Bahram; Turcatti, Gerardo; Marquet, Pierre (2018-09-10). "Quantification of stored red blood cell fluctuations by time-lapse holographic cell imaging". Biomedical Optics Express. 9 (10): 4714–4729. doi:10.1364/boe.9.004714. ISSN 2156-7085. PMC 6179419. PMID 30319898.
  31. ↑ "ChemBioFrance - Infrastructure de recherche". chembiofrance.cn.cnrs.fr. Retrieved 2021-01-05.
  32. ↑ "Innopharma" (in español). Retrieved 2021-01-05.
  33. ↑ "Members". European Cell-based Assays Interest Group. Retrieved 2021-01-05.
  34. ↑ "Combinatorial Chemistry & High Throughput Screening". Combinatorial Chemistry & High Throughput Screening. Retrieved 2021-01-05.

External links


Category:1959 births Category:Living people Category:University of the Republic (Uruguay) alumni Category:University of Geneva alumni Category:École Polytechnique Fédérale de Lausanne faculty Category:Scientists Category:Chemists


This article "Gerardo Turcatti" is from Wikipedia. The list of its authors can be seen in its historical and/or the page Edithistory:Gerardo Turcatti. Articles copied from Draft Namespace on Wikipedia could be seen on the Draft Namespace of Wikipedia and not main one.

Page kept on Wikipedia This page exists already on Wikipedia.

Retrieved from "https://en.everybodywiki.com/index.php?title=Gerardo_Turcatti&oldid=5313537"
License CC BY-SA 3.0
Powered by MediaWiki