ED1
ED1 is a monoclonal antibody clone directed against rat CD68, a 110 kD lysosomal glycoprotein and cellular marker specific for macrophages, Kupffer cells, osteoclasts, monocytes, and activated microglia.[1][2] [3] In the rat, CD68 is expressed in most macrophage populations and thus ED1 is widely used as a pan-macrophage marker. [4] In some cell types, it is detectable only when up-regulated, such as activated but not quiescent microglia, and can thus be used as a marker of inflammatory conditions and immune reactions in those instances. Commercial suppliers report that ED1 is used for detection of the CD68 protein by immunohistochemical staining, flow cytometry, and western blot methods and that in addition to rat it cross-reacts with bovine species.
The ED1 anti-CD68 antibody is not to be confused with the fibronectin extra domain ED1.[5]
References[edit]
- ↑ Dijkstra, CD; Döpp, EA; Joling, P; Kraal, G (1985). "The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3". Immunology. 54 (3): 589–599. PMC 1453512. PMID 3882559. Retrieved 12 September 2017.
- ↑ Gomes, LF; Lorente, S; Simon-Giavarotti, KA; Areco, KN; Araújo-Peres, C; Videla, LA (2004). "Tri-iodothyronine differentially induces Kupffer cell ED1/ED2 subpopulations". Molecular Aspects of Medicine. 25 (1–2): 183–190. doi:10.1016/j.mam.2004.02.018. PMID 15051326.
- ↑ Xie, R; Kuijpers-Jagtman, AM; Maltha, JC (2009). "Osteoclast differentiation and recruitment during early stages of experimental tooth movement in rats". European Journal of Oral Sciences. 117 (1): 43–50. doi:10.1111/j.1600-0722.2008.00588.x. PMID 19196317.
- ↑ Damoiseaux, JG; Döpp, EA; Calame, W; Chao, D; MacPherson, GG; Dijkstra, CD (1994). "Rat macrophage lysosomal membrane antigen recognized by monoclonal antibody ED1". Immunology. 83 (1): 140–147. PMC 1415006. PMID 7821959.
- ↑ Vincent, PA; Rebres, RA; Lewis, EP; Hurst, V; Saba, TM (1993). "Release of ED1 fibronectin from matrix of perfused lungs after vascular injury is independent of protein synthesis". American Journal of Physiology. 265 (5 Pt 1): L485–92. PMID 8238536.
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