Hollow fiber cell culture
The hollow fiber model is a cell culturing method entailing encapsulating cells in two cm long, 1 mm wide semipermeable PVDF-fibers[1]. The model was originally developed by National Cancer Institute (NCI) as a more high-throughput and less expensive alternative to the traditional xenograft models for anti cancer drug screening[2]. Hollow fibers filled with cells can be used for both In vitro and In vivo experimental procedures for example drug efficacy assays. Hollow fibers are filled with cells by using a syringe filled with cell suspension and separate fibers separated by heat-sealing. The hollow fibers can be cultured in vitro in petri-dishes or be implanted in animals and used in drug efficacy assays[1].
Other applications of hollow fibers[edit]
Hollow fibers without encapsulated cells can be used for different purposes, for example used as Hollow fiber membrane and Hollow fiber bioreactor.
==References
- ↑ 1.0 1.1 Hollingshead, Melinda G.; Alley, Michael C.; Camalier, Richard F.; Abbott, Betty J.; Mayo, Joseph G.; Malspeis, Louis; Grever, Michael R. (June 1995). "In vivo cultivation of tumor cells in hollow fibers". Life Sciences. 57 (2): 131–141. doi:10.1016/0024-3205(95)00254-4. ISSN 0024-3205. PMID 7603295.
- ↑ Decker, S.; Hollingshead, M.; Bonomi, C.A.; Carter, J.P.; Sausville, E.A. (April 2004). "The hollow fibre model in cancer drug screening". European Journal of Cancer. 40 (6): 821–826. doi:10.1016/j.ejca.2003.11.029. ISSN 0959-8049.
This article "Hollow fiber cell culture" is from Wikipedia. The list of its authors can be seen in its historical and/or the page Edithistory:Hollow fiber cell culture. Articles copied from Draft Namespace on Wikipedia could be seen on the Draft Namespace of Wikipedia and not main one.
